High throughput analysis and purification in support of automated parallel synthesis
Harold N. Weller
, Marian G. Young
Combinatorial Drug Discovery, Bristol-Myers Squibb Pharmaceutical
Research Institute, P.O.Box 4000, Princeton, NJ 08543-4000, U.S.A.
Stephen J. Michalczyk, Gary H. Reitnauer
Instrumen Development Group, Bristol-Myers Squibb Pharmaceutical
Research Institute, P.O.Box 4000, Princeton, NJ 08543-4000, U.S.A.
Robert S. Cooley, Peter C. Rahn, Dana J. Loyd
YMC Inc., 3233 Burnt Mill Drive, Wilmington, NC 28403, U.S.A.
Dario Fiore
, Steven J. Fischman
Shimadzu Scientific Instruments Inc., 7102 Riverwood Drive, Columbia,
MD 21046-2502, U.S.A.
Abstract
Rapid reverse-phase analytical and preparative HPLC methods have been
developed for aplication to parallel synthesis libraries. Gradient
methods, short columns, and high flow rates allow analysis of over 300
compounds per day on a single system, or purification of up to 200
compounds per day on a single preparative system. Hardware and software
modifications allow continuous unattended use for maximum efficiency and
throughput.
Keywords
combinatorial chemistry, combinatorial library, purification, HPLC,
preparative HPLC
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