High throughput analysis and purification in support of automated parallel synthesis
Harold N. Weller
, Marian G. Young
Combinatorial Drug Discovery, Bristol-Myers Squibb Pharmaceutical Research Institute, P.O.Box 4000, Princeton, NJ 08543-4000, U.S.A.
Stephen J. Michalczyk, Gary H. Reitnauer
Instrumen Development Group, Bristol-Myers Squibb Pharmaceutical Research Institute, P.O.Box 4000, Princeton, NJ 08543-4000, U.S.A.
Robert S. Cooley, Peter C. Rahn, Dana J. Loyd
YMC Inc., 3233 Burnt Mill Drive, Wilmington, NC 28403, U.S.A.
, Steven J. Fischman
Shimadzu Scientific Instruments Inc., 7102 Riverwood Drive, Columbia, MD 21046-2502, U.S.A.
Rapid reverse-phase analytical and preparative HPLC methods have been developed for aplication to parallel synthesis libraries. Gradient methods, short columns, and high flow rates allow analysis of over 300 compounds per day on a single system, or purification of up to 200 compounds per day on a single preparative system. Hardware and software modifications allow continuous unattended use for maximum efficiency and throughput.
combinatorial chemistry, combinatorial library, purification, HPLC, preparative HPLC
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