Filtration is Dead!

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Table of Contents

Filtration is Dead!

New Technique for High Throughput Synthesis

Compas 768.2 (Spyder Instruments, Inc.)

Separation of Solid & Liquid Phase

Centrifuge with Multiple Slingshots Leonardo da Vinci, Cod. Atl. F.57r.-b, 1488 (?)

Separation of Solid and Liquid Phase Principle of tilted centrifugation

Separation of Two Immiscible Liquid Phases Principle of tilted centrifugation extraction or fluorous phase synthesis

Applies to Wells of All Shapes

Microtiterplate Situation

Volume Considerations

Rotor With Adjustable Tilt

Contamination Issue

Is There a Danger of Bead Transfer? NO!

Compounds Synthesized in Neighboring Wells

Crude Model and First Prototype

PPT Slide

Multichannel Delivery of Solvents/Reagents

Compas 768.2

96 Channel Liquid Distributor

Detail of the Rotor

Internal or External Computer

Performing Individual Steps

Running Program

Setting Parameters

PPT Slide

Synthetic Center

Generating Compounds From Building Blocks

Editing Plate Layout

Protocol Editing

Program Creation

Plate Tracking

Program Tracking

Synthesis Algorithm

Mixing by Oscillation

Synthesis Algorithm (continuation)

HF Towers (Parallel cleavage in gas HF)

Result

Or

Or

Or

Is it Useful Today?

Example of Parallel Synthesis

Purity Evaluation (LC-MS TIC data)

LC-MS Evaluation of All Products

HPLCs of 768 Compounds Synthesized in One Batch

HPLCs of 768 Compounds Synthesized in One Batch

HPLCs of 768 Compounds Synthesized in One Batch

HPLCs of 768 Compounds Synthesized in One Batch

HPLCs of 768 Compounds Synthesized in One Batch

HPLCs of 768 Compounds Synthesized in One Batch

Is it Useful Today?

How About Miniaturization ?

Liquid Distribution

Liquid Removal

One Bead Per Well

Separation of Solid and Liquid Phase Principle of tilted centrifugation

Parallel Liquid-Liquid Extraction

Complete Removal of Liquid from Array of Wells Principle of tilted centrifugation plate washing

How About Tomorrow ? When and where can I get one of these instruments?

Spyder Instruments

Family of COMPAS Instruments Centrifugation-Based Techniques

Conclusions

Acknowledgement

Author: Vectra 5/166

Email: spyder@5z.com

Home Page: www.5z.com