Notes

Slide Show

Outline

1	"Centrifugation Based Automated Synthesis Technologies" Centrifugation Based Automated Synthesis Technologies
2	"Centrifugation:" Centrifugation: The Only Technique for Massively Parallel Solid Phase Synthesis
3	Filtration is Dead!
4	Solid Phase Synthesis
5	Solid Phase Synthesis
6	It All Started With… Jouan….
7	Or Did It All Start With…
8	Or Did It All Start With…
9	Actually It All Started With…
10	It All Started With Cotton
11	Centrifugal (Cotton) Veterans
12	CSPS
13	Continual Solid Phase Synthesis (CSPS)
14	Concept of Centrifugal Synthesizer
15	The First “Compas” (Cotton Multiple Peptide Synthesizer)
16	Spyder Instruments Started in 1992 in San Diego The first product - Compas 242 multiple automatic peptide synthesizer centrifugation inclusion volume coupling US Patents 5,202,418, 5,338,831, 5,342,585 SBIR Phase I and II grant recipient
17	Compas 242
18	Tilted Centrifugation
19	Tilted Centrifugation
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21	Studying Cross-Contamination Issue
22	Single Bead in a Well
23	No Cross Contamination
24	Microplate CFD Results Zero cross contamination
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33	Crude Model and First Prototype
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37	Second Generation Compas 768
38	Synthesis Algorithm Distribute resin (5 mg/well) into microtiterplates multichannel pipetting Place plates into COMPAS Distribute solutions by 96 channel distributor (Oscillate) Centrifuge Distribute building blocks and reagents multichannel pipetting or Multiprobe 104 Oscillate Centrifuge
39	Synthesis Algorithm (continuation) Remove protecting groups Wash plates in centrifuge Dry resin in plates in vacuum Place plates in HF chamber or distribute cleavage solution by 96 channel distributor Dry resin in plates in vacuum Extract product (reformat, take analytical samples) Evaporate or lyophilize product Seal microtiterplates
40	HF Chamber for Parallel Cleavage (“Bazooka”)
41	Betides - Examples of Results
42	Tetrahydroisoquinolinones
43	Tetrahydroisoquinolinones
44	How About Tomorrow ? When and where can I get one of these instruments? One COMPAS at each chemist’s bench modular approach simple centrifuge up to fully automatic machine from mg to kg scale Partner for commercialization ...
45	Illumina’s Revolutionary Technology: Bringing Fiber Optics to Biology
46	Fiber Bundles: Flexible Platform
47	Scalability of Technology
48	Custom Oligo Price History 1972 $1,667.00/base 1989 $ 10.00/base 1992 $ 3.50/base 1994 $ 2.19/base 1996 $ 1.50/base 1999 $ 0.75/base 2000 $ 0.60/base 2001 $ 0.24/base (Illumina) 2002 $ 0.16/base (Illumina)
49	First Generation Oligator^TM Prototype
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55	Carbon Fiber Rotor
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57	Third Generation of Oligator 768^TM
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60	From 4 L Flasks to 1250 L Drums
61	QC of Oligator Production: MS
62	QC of Oligator Production: HPLC
63	Coupling Efficiencies
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74	HPLC Traces of Unpurified 25-mer Oligos
75	HPLC Trace of Unpurified 25-mer Oligos
76	Commercial Interlude
77	The Oligator Farm^TMServices Custom oligo synthesis for large consumers Current capacity > 400M bases per year
78	We Are Shipping Oligos Daily!
79	Conclusions Tilted centrifugation is the most effective and simplest method for liquid removal and phase separation in multiplicity of vessels Polypropylene microtiterplates are ideal reaction vessels for tilted centrifugation based synthesis Tilted centrifugation is the only way for removal of liquids in ultraminiaturized techniques
80	"Centrifugation:" Centrifugation: The Only Technique for Massively Parallel Solid Phase Synthesis
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